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multi image light cabinet  (Alpha Innotech)
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Alpha Innotech multi image light cabinet
Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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multi image ii  (Alpha Innotech)
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Alpha Innotech multi image ii
Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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multi color chemiluminescence imaging system  (Alpha Innotech)
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Alpha Innotech multi color chemiluminescence imaging system
Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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alpha innotech multiimage iii  (Alpha Innotech)
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Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
Alpha Innotech Multiimage Iii, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multiimage scanning densitometer  (Alpha Innotech)
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Alpha Innotech multiimage scanning densitometer
Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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multiimager iii  (Alpha Innotech)
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Alpha Innotech multiimager iii
Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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chemiluminescence solution  (Alpha Innotech)
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Alpha Innotech chemiluminescence solution
Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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luminometer  (Cytiva Europe)
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Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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amersham ecl select western blot detection reagent  (Cytiva Europe)
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Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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chemiluminescence ecl  (Alpha Innotech)
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Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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immobilon western chemiluminescent hrp substrate  (Merck KGaA)
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Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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enhanced chemiluminescence detection kit  (Promega)
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Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a <t>Multi</t> <t>Image</t> <t>light</t> <t>cabinet</t> and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
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Image Search Results


Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a Multi Image light cabinet and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.

Journal: The American Journal of Pathology

Article Title: Expression and Potential Role of Fas-Associated Phosphatase-1 in Ovarian Cancer

doi: 10.1016/s0002-9440(10)64084-9

Figure Lengend Snippet: Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a Multi Image light cabinet and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.

Article Snippet: To quantify immunoblotting results, films were analyzed densitometrically using a Multi Image Light Cabinet and the ChemiImager software version 4000 (Alpha Innotech Corporation, San Leandro, CA).

Techniques: Comparison, SDS Page, Western Blot, Control, Software